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KMID : 0861020170320020065
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Korea Journal of Herbology
2017 Volume.32 No. 2 p.65 ~ p.75
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Orostachydis Herba and Fermented Orostachydis Herba Enhances Anti-Inflammatory and Anti-oxidant Effect against Lipopolysaccharide-Induced Acute Liver Injury in Mice
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Kang Han-Eun
Lee Ah-Reum
Roh Seong-Soo
Seo Young-Bae
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Abstract
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Objectives ： This study aimed to evaluate the protective effect of Orostachydis Herba (OH) and Fermented OH (OHF) against the acute liver injury by lipopolysaccharide (LPS).
Methods ： OHF by 4 lactic bacteria such as (Lactobacillus hilgardii (OHF1), Leuconostoc mesenteroides (OHF2), Pediococcus acidilactici (OHF3), Saccharomyces cerevisiae (OHF4)) were prepared. Samples were selected to OHF0, OHF2, OHF3 based on UPLC analysis, DPPH, ABTS radical scavenging activities. To evaluate the protective effect of OHF on liver injury mice, ICR mice were divided into 5 groups: Normal mice (Nor), LPS (20 ㎎/㎏) treated mice (Veh), administrated OHF0, OHF2 OHF3 200 ㎎/㎏ body weight during 8 days before LPS injection. Serum and liver were collected 24 hours after LPS injection.
Results ： The activity was high in order of OHF0 and OHF3 in DPPH and ABTS radical scavenging activities. The quercetin contents for bioactive ingredient of OH was 5.39, kaempferol contents was 9.94 by UPLC analysis. The LPStreated vehicle group significantly increased liver weight, and aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels in serum. In contrast, administrated OHF3 group decreased liver weight, AST, ALT. In addition, OHF3 groups reduced the elevated levels of reactive oxygen species (ROS) in serum and tissues. Moreover, AP-1, iNOS and COX-2 were significantly decreased in OHF2 and OHF3. But NF-κB p65 and TNF-α only showed a significant reduction in OHF3.
Conclusions ： Therefore, these results suggest that fermented Orostachydis Herba might be protective effect on liver injury through anti-oxidant effect.
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KEYWORD
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Orostachydis Herba, anti-oxidation, anti-inflammation, liver injury, lipopolysaccharide
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